microCLONE Tissue Culture Liquid Media, 120 ml
Tissueponics is microCLONE's liquid approach to tissue culture, built around small tubes instead of gelled agar plates. Each tube holds a little prepared medium and a single trimmed cutting. This bottle restocks that medium once the kit's original supply runs low. It arrives prepared and sterile, so there is no weighing of salts or cooking of agar. Inside is a balanced blend of sugar, nutrients and plant hormones. Cuttings take up all three directly through their surface while they sit in the liquid. The sugar acts as an energy source, so the cultures do not depend on bright light to grow. The hormones encourage branching and new shoots, turning one tip into several nodes. Those new nodes become the source of the next round of cuttings. microCLONE adds preservatives to reduce the chance of mold taking hold in the tubes. The 120 ml size suits a hobby grower running a small batch or topping up a few tubes at a time. A compact bottle also keeps less medium open between sessions.
Running a Tissueponics Refill Through a Fresh Batch
Set up a clean work area first, with a HEPA filter moving air across the bench. Sanitize the glass culture tubes before use, since the tubes in the kit are not sterile. The kit's Dichlor sanitizer is made for that step. Wipe hands, tools and the outside of the bottle with alcohol before opening it. Pour a small amount of medium into each tube, enough to reach the base of the cutting. Trim limb tips from the strongest mother plants and drop one into each tube. Seal the tubes with the kit tape and move them to a shelf under a low wattage grow light. The Tissueponics method runs about three weeks from cutting to rooting plug. Check the tubes every few days, and pull any that turn cloudy or show fuzzy growth. Once branches form, transfer the tube cuttings straight into rooting plugs. Cap the bottle tightly after every pour to keep the remaining medium clean.
Key Features
- Sterile and Prepared: Ready to pour into tubes with no mixing or cooking.
- Sugar Energy Source: Feeds cultures without needing bright light.
- Branching Hormones: Encourages new shoots and nodes for more cuttings.
- Nutrient Blend: Absorbed directly through the cutting surface.
- Mold Preservatives: Added to lower the chance of contamination.
- 120 ml Bottle: Compact size for small batches and top ups.
Technical Specifications
| Specification | Value |
|---|---|
| Brand | microCLONE |
| SKU | PF3011 |
| Product | Liquid media refill for Tissueponics kits |
| Volume | 120 ml |
| Form | Prepared sterile liquid medium |
| Contents | Sugar, nutrients and plant hormones, with preservatives |
| Use | Liquid tissue culture in culture tubes |