What the microCLONE Multiplication Media Kit BAP Is Built On
Benzylaminopurine at 1 mg per liter is the workhorse cytokinin of plant tissue culture, and that is what this kit carries. Underneath it sits 4.43 g of MS nutrient and vitamins plus 1 mL of PPM preservative in the 60 mL concentrate. The 4 g gellan gum and agar mix is optional here, so liquid and gelled protocols are both on the table.
Running Establishment and Multiplication on One Medium
This is the medium most protocols start on and stay on the longest, holding shoot lines while they build up subculture after subculture. Where cultures stall on it, growers step across to the thidiazuron version rather than pushing the dose here. Shoots multiplied under any cytokinin still need a rooting medium afterwards. Stage the rooting kit alongside this one.
Key Features
- 1 mg BAP per liter: The standard multiplication rate, mild enough to subculture on repeatedly without distortion.
- 4.43 g MS nutrient and vitamins: Murashige and Skoog salts with vitamins, the same base as the rest of the series.
- 1 mL PPM preservative: In-vessel protection that supports sterile technique rather than replacing it.
- Optional gellan gum and agar: 4 g proprietary mix included as an option, so liquid protocols are not forced to gel.
- Makes one liter: One 60 mL concentrate per liter, which keeps batch math simple across a run of vessels.
Technical Specifications
| Specification | Value |
|---|---|
| SKU | PF3001 |
| Stage | Establishment and multiplication |
| Liquid Component | 60 mL concentrate |
| Base | 4.43 g MS nutrient and vitamins |
| Hormone | 1 mg BAP |
| Preservative | 1 mL PPM |
| Gelling Agent | 4 g gellan gum and agar mix (optional) |
| Yield | 1 liter of finished media |