Why the microCLONE Multiplication Media Kit TDZ Uses Thidiazuron
TDZ is a far stronger cytokinin than the benzylaminopurine most multiplication media use. That is why the dose here is 0.3 mg rather than a whole milligram. It sits on 4.43 g of MS nutrients and vitamins with 1 mL of PPM preservative. Four grams of the proprietary gellan gum and agar mix comes in the box. The kit is labeled for introduction and multiplication work.
Choosing a TDZ Medium Over the BAP Version
Difficult species and stubborn explants are where thidiazuron earns its place, since it drives shoot proliferation when milder cytokinins stall. That strength cuts both ways: overexposure gives you vitrified, fused shoots that will not root cleanly later. Cultures normally move off it onto a milder multiplication medium before rooting, so treat it as a stage rather than a home.
Key Features
- 0.3 mg TDZ per liter: A low dose by design, because thidiazuron works at a fraction of a benzylaminopurine rate.
- 4.43 g MS nutrient and vitamins: The same Murashige and Skoog base used across the microCLONE media series.
- Introduction and multiplication: Labeled for both stages, which covers explants coming in and shoot lines building up.
- 1 mL PPM preservative: Contamination protection inside the vessel, working alongside sterile technique.
- Gellan gum and agar included: 4 g of the proprietary mix ships with the kit rather than being sold on the side.
Technical Specifications
| Specification | Value |
|---|---|
| SKU | PF3002 |
| Stage | Introduction and multiplication |
| Liquid Component | 60 mL concentrate |
| Base | 4.43 g MS nutrient and vitamins |
| Hormone | 0.3 mg TDZ |
| Preservative | 1 mL PPM |
| Gelling Agent | 4 g gellan gum and agar mix |
| Yield | 1 liter of finished media |