What Goes Into a microCLONE Rooting Media Kit
The 60 mL liquid component holds 4.43 g of MS nutrient and vitamins, 1 mg IBA, 0.5 mg NAA and 1 mL of PPM preservative. Two auxins rather than one is the point of a rooting formulation, since IBA and NAA push root initiation from different angles. A 4 g proprietary gellan gum and agar mix comes with the kit as an option for anyone gelling rather than running liquid.
Where the Rooting Stage Sits in a Tissue Culture Run
This is the third medium in a normal sequence, after establishment and multiplication have done their work. Cultures move onto it once there is enough shoot material to root out. It is the step that decides how many make it to soil. The PPM in the mix is a preservative, not a substitute for sterile technique. The flow hood and the autoclave still do the heavy lifting.
Key Features
- 4.43 g MS nutrient and vitamins: The Murashige and Skoog base that most plant tissue culture work is built on.
- 1 mg IBA and 0.5 mg NAA: Two auxins working together on root initiation rather than a single hormone doing it alone.
- 1 mL PPM preservative: Broad spectrum protection in the vessel, alongside sterile technique rather than instead of it.
- Makes one liter: Each 60 mL concentrate mixes out to a full liter of finished rooting medium.
- Gellan gum and agar included: A 4 g proprietary mix supplied as an option for gelled media rather than liquid culture.
Technical Specifications
| Specification | Value |
|---|---|
| SKU | PF3003 |
| Stage | Rooting |
| Liquid Component | 60 mL concentrate |
| Base | 4.43 g MS nutrient and vitamins |
| Hormones | 1 mg IBA, 0.5 mg NAA |
| Preservative | 1 mL PPM |
| Gelling Agent | 4 g gellan gum and agar mix (optional) |
| Yield | 1 liter of finished media |